An Improved Method for the Determination of the Plasma Volume with Evans Blue

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The extraction of Evans blue (T1824) from plasma and the measurement of plasma volume.

Haemolysis and lipaemia interfere with the direct photometric estimation of Evans blue in plasma. During an investigation of blood volume changes in surgical shock using this dye, lipaemia was often encountered and it was necessary to use an extraction procedure for the estimation of the dye concentration. A rapid method was desired. The method of Crooke and Morris (1942) using a hydrochloric a...

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The methods at present available for the determination of the dye, Evans blue, in plasma have certain disadvantages. Direct colorimetry of dyed plasma is inapplicable where the plasma is haemolyzed, turbid or lipaemic. The butanol extraction procedure of Harington, Pochin & Squire (1940) is troublesome and time-consuming where many analyses must be carried out; its accuracy is lower than those ...

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ژورنال

عنوان ژورنال: Clinical Chemistry and Laboratory Medicine

سال: 1981

ISSN: 1434-6621,1437-4331

DOI: 10.1515/cclm.1981.19.9.919